Circular RNA MAN2B2 promotes cell proliferation of hepatocellular carcinoma cells via the miRNA-217/MAPK1 axis

Fu, Xiaoying; Zhang, Juanjuan; He, Xing; Yan, Xu; Wei, Jian; Huang, Min; Liu, Yaya; Lin, Jianwei; Hu, Hongxing; liu, Lei

doi:10.7150/jca.36500

International Journal of Biological Sciences

International Journal of Medical Sciences

Theranostics

Journal of Genomics

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J Cancer 2020; 11(11):3318-3326. doi:10.7150/jca.36500 This issue Cite

Research Paper

Circular RNA MAN2B2 promotes cell proliferation of hepatocellular carcinoma cells via the miRNA-217/MAPK1 axis

Xiaoying Fu^1,2#, Juanjuan Zhang^1#, Xing He^1#, Xu Yan¹, Jian Wei¹, Min Huang¹, Yaya Liu¹, Jianwei Lin^3✉, Hongxing Hu^1✉, Lei liu^1✉

1. Shenzhen Third People's Hospital, Second Affiliated Hospital of Southern University of Science and Technology.
2. Shenzhen University.
3. Shenzhen People's Hospital. Shenzhen 518039, China
# Equal contribution

Citation:

Fu X, Zhang J, He X, Yan X, Wei J, Huang M, Liu Y, Lin J, Hu H, liu L. Circular RNA MAN2B2 promotes cell proliferation of hepatocellular carcinoma cells via the miRNA-217/MAPK1 axis. J Cancer 2020; 11(11):3318-3326. doi:10.7150/jca.36500. https://www.jcancer.org/v11p3318.htm

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Abstract

The increasing incidence of hepatocellular carcinoma (HCC) is a major challenge worldwide. In the past few years, an increasing number of studies have suggested that circular RNAs (circRNAs) play an important role in the development of human tumors, including HCC, but our understanding of their function is still limited. In this study, we investigated differences in the expression of circRNA MAN2B2 (circMAN2B2) in hepatocellular tissues and paired normal tissues. We found that knockdown of circMAN2B2 expression in the HCC cell lines Hep-G2 and Huh-7 significantly inhibited cell proliferation by sponging (miRNA) miR-217 and inhibiting its function. Through a series of experiments, we also demonstrated that miR-217 functioned as a tumor suppressor molecule in HCC cells and regulated the expression of mitogen-activated protein kinase 1 (MAPK1). Restoration of MAPK1 rescued repression of cell proliferation induced by circMAN2B2 knockdown. In summary, our study indicated that circMAN2B2 acted as an onco-miRNA in HCC by sponging miRNA-217 to promote MAPK1 expression.

Keywords: hepatocellular carcinoma, circMAN2B2, miR-217, MAPK1, circular RNA

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APA

Fu, X., Zhang, J., He, X., Yan, X., Wei, J., Huang, M., Liu, Y., Lin, J., Hu, H., liu, L. (2020). Circular RNA MAN2B2 promotes cell proliferation of hepatocellular carcinoma cells via the miRNA-217/MAPK1 axis. Journal of Cancer, 11(11), 3318-3326. https://doi.org/10.7150/jca.36500.

ACS

Fu, X.; Zhang, J.; He, X.; Yan, X.; Wei, J.; Huang, M.; Liu, Y.; Lin, J.; Hu, H.; liu, L. Circular RNA MAN2B2 promotes cell proliferation of hepatocellular carcinoma cells via the miRNA-217/MAPK1 axis. J. Cancer 2020, 11 (11), 3318-3326. DOI: 10.7150/jca.36500.

NLM

CSE

Fu X, Zhang J, He X, Yan X, Wei J, Huang M, Liu Y, Lin J, Hu H, liu L. 2020. Circular RNA MAN2B2 promotes cell proliferation of hepatocellular carcinoma cells via the miRNA-217/MAPK1 axis. J Cancer. 11(11):3318-3326.

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