High expression of folate cycle enzyme MTHFD1L correlates with poor prognosis and increased proliferation and migration in colorectal cancer

He, Zhongyun; Wang, Xia; Zhang, Huizhong; Liang, Baoxia; Zhang, Jinling; Zhang, Zhenfeng; Yang, Yi

doi:10.7150/jca.35014



International Journal of Biological Sciences

International Journal of Medical Sciences

Theranostics

Journal of Genomics

Nanotheranostics

Global reach, higher impact

Full Text | PDF

J Cancer 2020; 11(14):4213-4221. doi:10.7150/jca.35014 This issue Cite

Research Paper

High expression of folate cycle enzyme MTHFD1L correlates with poor prognosis and increased proliferation and migration in colorectal cancer

Zhongyun He^1*, Xia Wang^1*, Huizhong Zhang³, Baoxia Liang², Jinling Zhang², Zhenfeng Zhang^2✉, Yi Yang^1,4✉

1. Department of Pathology, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, PR, China.
2. Department of Radiology, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, PR, China.
3. Department of Pathology, Sun Yat-sen University Cancer Center, Guangzhou, PR, China.
4. Boba Evergrande International Hospital, Qionghai, PR, China.
*Zhongyun He and Xia Wang contributed equally to this work.

✉ Corresponding authors: Yi Yang, Department of Pathology, The Second Affiliated Hospital of Guangzhou Medical University, 250 Changgang Rd East, Guangzhou, China. Boao Evergrande International Hospital, Kangxiang Road, Boao Lecheng Medical Tourism Pi More

Citation:

He Z, Wang X, Zhang H, Liang B, Zhang J, Zhang Z, Yang Y. High expression of folate cycle enzyme MTHFD1L correlates with poor prognosis and increased proliferation and migration in colorectal cancer. J Cancer 2020; 11(14):4213-4221. doi:10.7150/jca.35014. https://www.jcancer.org/v11p4213.htm

Other styles

Abstract

Aims: To investigate the expression and clinical significance of methylenetetrahydrofolate dehydrogenase 1-like (MTHFD1L) in colorectal cancer (CRC) and its effect on CRC cells proliferation and migration.

Methods: 59 fresh CRC tissue samples and matched normal tissues, 176 archive CRC tissue samples and 8 CRC cell lines were tested MTHFD1L by western blot and immunohistochemistry, respectively. The relationship between MTHFD1L expression, clinical significance and prognosis was analyzed by chi-square test and survival analysis. MTT assay, plate clonal formation assay and scratch assay were used to verify the effect of MTHFD1L on the proliferation and migration in CRC cell lines.

Results: The results showed that the protein level of MTHFD1L in CRC was significantly higher than that in adjacent normal tissues (p<0.01). The expression of MTHFD1L in CRC was positively correlated with the degree of tumor differentiation, TNM classification, tumor invasion, lymph node metastasis, and distant metastasis. Survival analysis showed that CRC patients with high MTHFD1L expression had a lower 5-year survival rate and the expression of MTHFD1L was an independent adverse factor for the CRC prognosis (p<0.05). Down-regulation of MTHFD1L inhibited the proliferation and migration of DLD-1 and HCT116 CRC cell lines.

Conclusion: These findings reveal that MTHFD1L is highly expressive in CRC and associated with poor prognosis, and MTHDF1L can increase colorectal cancer cell proliferation and migration. Therefore, MTHFD1L may serve as a predictor and a potential therapeutic target for CRC.

Keywords: colorectal cancer, MTHFD1L, prognosis, migration, proliferation

Citation styles

APA

He, Z., Wang, X., Zhang, H., Liang, B., Zhang, J., Zhang, Z., Yang, Y. (2020). High expression of folate cycle enzyme MTHFD1L correlates with poor prognosis and increased proliferation and migration in colorectal cancer. Journal of Cancer, 11(14), 4213-4221. https://doi.org/10.7150/jca.35014.

ACS

He, Z.; Wang, X.; Zhang, H.; Liang, B.; Zhang, J.; Zhang, Z.; Yang, Y. High expression of folate cycle enzyme MTHFD1L correlates with poor prognosis and increased proliferation and migration in colorectal cancer. J. Cancer 2020, 11 (14), 4213-4221. DOI: 10.7150/jca.35014.

NLM

CSE

He Z, Wang X, Zhang H, Liang B, Zhang J, Zhang Z, Yang Y. 2020. High expression of folate cycle enzyme MTHFD1L correlates with poor prognosis and increased proliferation and migration in colorectal cancer. J Cancer. 11(14):4213-4221.

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.