J Cancer 2020; 11(16):4754-4761. doi:10.7150/jca.33527 This issue

Research Paper

Bovine Interferon-Tau Activates Type I interferon-Associated Janus-signal Transducer in HPV16-positive Tumor Cell

Geny Fierros-Zárate1, Clarita Olvera2, Gustavo Salazar-Guerrero1, Ausencio Morales-Ortega1, Fernando Reyna1, Eva Hernández-Márquez1, Eduardo Guzmán-Olea1,3, Ana I. Burguete-García1, Vicente Madrid-Marina1, Oscar Peralta-Zaragoza1, Marilú Chávez-Castillo1,4, Víctor Hugo Bermúdez-Morales1✉

1. Centro de Investigación sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca Morelos, México.
2. Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca Morelos, México.
3. Catedrático Consejo Nacional de Ciencia y Tecnología (CONACYT). Instituto de Ciencias de la Salud (ICSA), Universidad Autónoma del Estado de Hidalgo (UAEH), México.
4. Universidad Tecnológica Emiliano Zapata de Morelos, UTEZ.

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Citation:
Fierros-Zárate G, Olvera C, Salazar-Guerrero G, Morales-Ortega A, Reyna F, Hernández-Márquez E, Guzmán-Olea E, Burguete-García AI, Madrid-Marina V, Peralta-Zaragoza O, Chávez-Castillo M, Bermúdez-Morales VH. Bovine Interferon-Tau Activates Type I interferon-Associated Janus-signal Transducer in HPV16-positive Tumor Cell. J Cancer 2020; 11(16):4754-4761. doi:10.7150/jca.33527. Available from https://www.jcancer.org/v11p4754.htm

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Abstract

The mechanisms of signal transduction by interferon-tau (IFN-τ) are widely known during the gestation of ruminants. In trophoblast cells, IFN-τ involves the activation of the JAK-STAT pathway, and it can have effects on other cell types, such as tumor cells. Here we report that the HPV16-positive BMK-16/myc cell treated with ovine IFN-τ, results in the activation of the canonical JAK-STAT and non-canonical JAK-STAT pathway. The MAPK signaling pathway was activated, we detected the proteins MEK1, MEK2, Raf1, STAT3, STA4, STAT5 and STAT6. Moreover, IFN-τ induced the expression of MHC Class I, MX and IP10 in the tumor cells and this response may be associated with the viral replication and with the anti-proliferative and the immunoregulatory effects of IFN-τ.

Keywords: IFN-τ, type 1 interferon, signal transducer, BMK-16/myc, tumor cells