J Cancer 2020; 11(19):5768-5781. doi:10.7150/jca.44573 This issue

Research Paper

A novel seven-gene signature as Prognostic Biomarker in Hepatocellular Carcinoma

Hui Xie1,2,3,4*, Shouping Liu1,2,3*, Ziying Zhang5, Peng Chen6, Yongguang Tao1,2,3,4,6✉

1. Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University.
2. Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Department of Pathology, Xiangya Hospital, Central South University, Hunan, 410078 China.
3. NHC Key Laboratory of Carcinogenesis (Central South University), Cancer Research Institute and School of Basic Medicine, Central South University, Changsha, Hunan, 410078 China.
4. Department of Thoracic Surgery, Hunan Key Laboratory of Early Diagnosis and Precision Therapy in Lung Cancer, Second Xiangya Hospital, Central South University, Changsha, 410011 China.
5. Department of Oncology, Third Xiangya Hospital, Central South University, Changsha, Hunan, 410013, China.
6. Department of Urology, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.
*These authors contributed equally to this work.

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Xie H, Liu S, Zhang Z, Chen P, Tao Y. A novel seven-gene signature as Prognostic Biomarker in Hepatocellular Carcinoma. J Cancer 2020; 11(19):5768-5781. doi:10.7150/jca.44573. Available from https://www.jcancer.org/v11p5768.htm

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Purpose: Our study is designed to develop and certify a promising prognostic signature for hepatocellular carcinoma (HCC).

Materials and methods: We retrospectively analyzed mRNA expression profiles and clinicopathological data fetched from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets. We formulated a prognostic seven-gene signature composed of differentially expressed mRNAs (DEmRNAs) between HCC and nonneoplastic tissues through univariate Cox regression analysis. The receiver operating characteristic (ROC) curve, survival analysis and multivariate Cox regression analysis as well as nomograms were utilized to assess the prognostic performance of the seven-gene signature.

Results: The risk score based on a seven-gene signature categorized HCC subjects into a high- and low-risk group. There was significantly discrepant overall survival (OS) between patients in both groups and the corresponding ROC curve revealed a satisfactory predictive performance in HCC survival in both TCGA and GSE76427 cohort. Multivariate Cox regression analysis demonstrated that a seven-gene signature was an independently prognostic factor for HCC. Nomograms combining this prognostic signature with significant clinical characteristics conferred a crucial reference to predict the 1-,3- and 5 years OS.

Conclusions: Our study defined a promising seven-gene signature and nomogram model to forecast the OS of HCC patients, which is instrumental in clinical decision and personalized therapy.

Keywords: hepatocellular carcinoma, prognosis, risk score, prognostic signature