J Cancer 2021; 12(7):1978-1989. doi:10.7150/jca.50897 This issue Cite

Research Paper

Comparison of Proteomics Profiles Between Xenografts Derived from Cell Lines and Primary Tumors of Thyroid Carcinoma

Luo Fang2*, Yu-jia Liu3*, Yi-wen Zhang3,4, Zong-fu Pan3,4, Li-ke Zhong2, Lie-hao Jiang1,4, Jia-feng Wang1,4, Xiao-wei Zheng3, Ling-ya Chen2, Ping Huang3,4, Ming-hua Ge1,4, Zhuo Tan1,4✉

1. Department of Head and neck & thyroid surgery, Zhejiang Provincial People's Hospital; People's Hospital of Hangzhou Medical College, 310010, Hangzhou, Zhejiang, China.
2. Department of Pharmacy, The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, 1# Bashan East Road, 310022, Hangzhou, China.
3. Department of Pharmacy, Zhejiang Provincial People's Hospital; People's Hospital of Hangzhou Medical College, 310010, Hangzhou, Zhejiang, China.
4. Key Laboratory of Endocrine Gland Diseases of Zhejiang Province, 310010, Hangzhou, Zhejiang, China.
* Luo Fang and Yujia Liu contributed equally to this work.

Citation:
Fang L, Liu Yj, Zhang Yw, Pan Zf, Zhong Lk, Jiang Lh, Wang Jf, Zheng Xw, Chen Ly, Huang P, Ge Mh, Tan Z. Comparison of Proteomics Profiles Between Xenografts Derived from Cell Lines and Primary Tumors of Thyroid Carcinoma. J Cancer 2021; 12(7):1978-1989. doi:10.7150/jca.50897. https://www.jcancer.org/v12p1978.htm
Other styles

File import instruction

Abstract

Graphic abstract

Patient-consistent xenograft model is a challenge for all cancers but particularly for thyroid cancer, which shows some of the greatest genetic divergence between human tumors and cell lines. In this study, proteomic profiles of tumor tissues from patients, included anaplastic thyroid carcinoma (ATC) and papillary thyroid carcinoma, and xenografts (8305C, 8505C, FRO, BAPAP and IHH4) were obtained using HPLC-tandem mass spectrometry and compared based on all proteins detected (3,961), cancer-related proteins and druggable proteins using pairwise Pearson's correlation analysis. The human tissue showed low proteomic similarity to the ATC cell lines (8305C, r = 0.344-0.416; 8505C, 0.47-0.579; FRO, 0.267-0.307) and to PTC cell lines (BCPAP, 0.303-0.468; IHH4, 0.262-0.509). Human tissue showed the following similarity to cell lines at the level of 135 cancer-related pathways. The ATC cell lines contained 47.4% of the cancer-related pathways (19.26%-33.33%), while the PTC cell lines contained 40% (BCPAP, 25.93%; IHH4, 28.89%). In patient tumor tissues, 44-60 of 76 and 52-53 of 93 druggable proteins were identified in ATC and PTC tumors, respectively. Ten and 29 druggable proteins were not identified in any of the ATC and PTC xenografts, respectively. We provide a reference for CDX selecting in in vivo studies of thyroid cancer.

Keywords: Proteomics, Cell-line Derived Xenografts, Primary Tumor, Thyroid Cancer.


Citation styles

APA
Fang, L., Liu, Y.j., Zhang, Y.w., Pan, Z.f., Zhong, L.k., Jiang, L.h., Wang, J.f., Zheng, X.w., Chen, L.y., Huang, P., Ge, M.h., Tan, Z. (2021). Comparison of Proteomics Profiles Between Xenografts Derived from Cell Lines and Primary Tumors of Thyroid Carcinoma. Journal of Cancer, 12(7), 1978-1989. https://doi.org/10.7150/jca.50897.

ACS
Fang, L.; Liu, Y.j.; Zhang, Y.w.; Pan, Z.f.; Zhong, L.k.; Jiang, L.h.; Wang, J.f.; Zheng, X.w.; Chen, L.y.; Huang, P.; Ge, M.h.; Tan, Z. Comparison of Proteomics Profiles Between Xenografts Derived from Cell Lines and Primary Tumors of Thyroid Carcinoma. J. Cancer 2021, 12 (7), 1978-1989. DOI: 10.7150/jca.50897.

NLM
Fang L, Liu Yj, Zhang Yw, Pan Zf, Zhong Lk, Jiang Lh, Wang Jf, Zheng Xw, Chen Ly, Huang P, Ge Mh, Tan Z. Comparison of Proteomics Profiles Between Xenografts Derived from Cell Lines and Primary Tumors of Thyroid Carcinoma. J Cancer 2021; 12(7):1978-1989. doi:10.7150/jca.50897. https://www.jcancer.org/v12p1978.htm

CSE
Fang L, Liu Yj, Zhang Yw, Pan Zf, Zhong Lk, Jiang Lh, Wang Jf, Zheng Xw, Chen Ly, Huang P, Ge Mh, Tan Z. 2021. Comparison of Proteomics Profiles Between Xenografts Derived from Cell Lines and Primary Tumors of Thyroid Carcinoma. J Cancer. 12(7):1978-1989.

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
Popup Image