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J Cancer 2021; 12(19):5838-5847. doi:10.7150/jca.57926 This issue Cite

Research Paper

18F-FDG PET/CT metabolic parameters correlate with EIF2S2 expression status in colorectal cancer

Jian-Wei Yang1,2*, Ling-Ling Yuan3*, Yan Gao2, Xu-Sheng Liu2, Yu-Jiao Wang6, Lu-Meng Zhou1,2, Xue-Yan Kui1,2, Xiao-Hui Li2, Chang-Bin Ke2✉, Zhi-Jun Pei1,2,4,5✉

1. Postgraduate Training Basement of Jinzhou Medical University, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
2. Department of Nuclear Medicine and Institute of Anesthesiology and Pain, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
3. Department of Pathology, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
4. Hubei Key Laboratory of Embryonic Stem Cell Research, Shiyan, Hubei, China.
5. Hubei Key Laboratory of WudangLocal Chinese Medicine Research, Shiyan, Hubei, China.
6. Department of Radiology, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang, Hubei, China.
*Contributed equally to this work.

✉ Corresponding authors: Zhijun Pei, E-mail: pzjun1980net; Changbin Ke, E-mail: changbinke-iapcom.

Citation:
Yang JW, Yuan LL, Gao Y, Liu XS, Wang YJ, Zhou LM, Kui XY, Li XH, Ke CB, Pei ZJ. 18F-FDG PET/CT metabolic parameters correlate with EIF2S2 expression status in colorectal cancer. J Cancer 2021; 12(19):5838-5847. doi:10.7150/jca.57926. https://www.jcancer.org/v12p5838.htm
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Abstract

Graphic abstract

Background: We sought to investigate whether the expression of the gene EIF2S2 is related to 18F-FDG PET/CT metabolic parameters in patients with colorectal cancer (CRC).

Materials and methods: The expression of EIF2S2 in CRC and its relationship with clinicopathological features were obtained through the ONCOMINE, UALCAN and GEPIA databases. EIF2S2 and GLUT1 expression were examined by immunohistochemistry in 42 CRC patients undergoing preoperative PET-CT examination. Spearman correlation analysis was used to assess the relationship between EIF2S2 and GLUT1 levels and clinical parameters. Correlation analysis between EIF2S2 and Reactome-Glycolysis signatures was performed using GEPIA2. We describe the effect of EIF2S2 knockdown on lactate production and the mRNA levels of glycolysis-related genes in human colon cancer SW480 cells.

Results: Immunohistochemistry revealed an upregulation of EIF2S2 protein expression in tumor tissues of colorectal cancer patients, which is consistent with the significant upregulation of EIF2S2 transcript levels in the database. These colorectal cancer patients included 24 cases of colon cancer and 18 cases of rectal cancer, ranging in age from 31 to 78 years. The transcription was significantly related to histological subtypes and TP53 mutations (P <0.05). The value of SUVmax in CRC significantly correlated with the expression of EIF2S2 (rho = 0.462, P <0.01). Although SUVmax and SUVmean was not correlate with the expression of GLUT1 (P <0.05), a significant correlation was observed between the expression of GLUT1 and the volumetric PET parameters, such as MTV and TLG (P < 0.01). GLUT1 expression in CRC was positively correlated with EIF2S2 status (rho = 0.470, P <0.01). In SW480 cells, RNAi-mediated depletion of EIF2S2 inhibited lactic acid production (P <0.05) and SLC2A1, SLC2A3, SLC2A10, HK2, PKM2, LDHA mRNA level (P <0.01).

Conclusions: Primary CRC FDG uptake is strongly associated with the overexpression of EIF2S2, and EIF2S2 may promote glycolysis in CRC by mediating GLUT1.

Keywords: Colorectal cancer, EIF2S2, 18F-FDG, SUVmax, glucose transporter 1

Citation styles

APA
Yang, J.W., Yuan, L.L., Gao, Y., Liu, X.S., Wang, Y.J., Zhou, L.M., Kui, X.Y., Li, X.H., Ke, C.B., Pei, Z.J. (2021). 18F-FDG PET/CT metabolic parameters correlate with EIF2S2 expression status in colorectal cancer. Journal of Cancer, 12(19), 5838-5847. https://doi.org/10.7150/jca.57926.

ACS
Yang, J.W.; Yuan, L.L.; Gao, Y.; Liu, X.S.; Wang, Y.J.; Zhou, L.M.; Kui, X.Y.; Li, X.H.; Ke, C.B.; Pei, Z.J. 18F-FDG PET/CT metabolic parameters correlate with EIF2S2 expression status in colorectal cancer. J. Cancer 2021, 12 (19), 5838-5847. DOI: 10.7150/jca.57926.

NLM
Yang JW, Yuan LL, Gao Y, Liu XS, Wang YJ, Zhou LM, Kui XY, Li XH, Ke CB, Pei ZJ. 18F-FDG PET/CT metabolic parameters correlate with EIF2S2 expression status in colorectal cancer. J Cancer 2021; 12(19):5838-5847. doi:10.7150/jca.57926. https://www.jcancer.org/v12p5838.htm

CSE
Yang JW, Yuan LL, Gao Y, Liu XS, Wang YJ, Zhou LM, Kui XY, Li XH, Ke CB, Pei ZJ. 2021. 18F-FDG PET/CT metabolic parameters correlate with EIF2S2 expression status in colorectal cancer. J Cancer. 12(19):5838-5847.

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