J Cancer 2021; 12(24):7380-7389. doi:10.7150/jca.62112 This issue

Research Paper

Linc01513 inhibits the malignant potential of Nasopharyngeal carcinoma by binding to PTBP1

Juan Wang1,2✉, Xiaolan Cai1,2, Liqiang Zhang1,2, Dapeng Lei1,2✉

1. Department of Otorhinolaryngology, Qilu Hospital of Shandong University, Jinan, China
2. NHC Key Laboratory of Otorhinolaryngology, Shandong University, Jinan, China

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Citation:
Wang J, Cai X, Zhang L, Lei D. Linc01513 inhibits the malignant potential of Nasopharyngeal carcinoma by binding to PTBP1. J Cancer 2021; 12(24):7380-7389. doi:10.7150/jca.62112. Available from https://www.jcancer.org/v12p7380.htm

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Abstract

Graphic abstract

LncRNAs are reported to be involved in tumor proliferation, invasion and metastasis, and are considered as potential biomarkers and therapeutic targets for human cancer, including head and neck cancer. In this study, we screened the differentially low-expressed linc01513 by bioinformatic to detect its expression and biological effect on nasopharyngeal carcinoma (NPC). MTT was used to evaluate the effect of linc01513 on the proliferation of NPC cells. Wound healing assay was used to determine the cells migration ability. The matrix transwell was used to further detect the role of linc01513 in cell invasion. Western blot was used to detect the expression of epithelial-mesenchymal transformation (EMT)-induced transcription factors E-cadherin, vimentin and Slug. The results showed that silence of linc01513 could promoted the proliferation, migration and invasion of NPC cells. The in vivo experiment showed that overexpression of linc01513 could inhibit the volume and weight of xenograft tumors. Database prediction, RNA pull-down and RIP experiments suggested that linc01513 may play an anti-tumor effect by inhibiting PTBP1 protein level. It is suggested that linc01513 directly binds to PTBP1 protein and mediates the EMT process and malignant biological behavior of NPC cells, which provides a new molecular marker for the prognosis and treatment of NPC.

Keywords: linc01513, Nasopharyngeal carcinoma, proliferation, invasion, PTBP1