J Cancer 2021; 12(24):7507-7517. doi:10.7150/jca.64940 This issue

Research Paper

Prognostic value of LINC02560 in colorectal cancer correlates with tumor microenvironment immunity

Chunying Luo1,2*, Fahui Liu2*, Weichao Su3*, Puze Long2*, Jiadong Liang2, Wanyun Hou2, Haifeng Jiang3, Xidai Long2, Guoqiang Su1,3✉

1. Department of Cell Biology, Medical College of Guangxi University, Nanning 530004, Guangxi, PR China.
2. Department of Pathology, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise 533000, Guangxi, PR China.
3. Department of Gastrointestinal Surgery III, Xiamen Cancer Hospital, First Affiliated Hospital of Xiamen University, 55 Zhenhai Road, Xiamen 361003, China.
*These authors contributed equally to this work.

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Citation:
Luo C, Liu F, Su W, Long P, Liang J, Hou W, Jiang H, Long X, Su G. Prognostic value of LINC02560 in colorectal cancer correlates with tumor microenvironment immunity. J Cancer 2021; 12(24):7507-7517. doi:10.7150/jca.64940. Available from https://www.jcancer.org/v12p7507.htm

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Abstract

Graphic abstract

Background: LINC02560 is a new 477 bp long non-coding RNA located in 19q13.43. However, the expression of LINC02560 in colorectal cancer (CRC) has not been reported, and its correlation with tumor development and function is still unclear.

Methods: The expression of LINC02560 in CRC was first analyzed in the cancer genome atlas (TCGA) combined with The Genotype-Tissue Expression(GTEx) databases and then validated by clinical CRC samples and cell lines. The association between LINC02560 expression and clinicopathologic variables was analyzed by the Wilcoxon Rank SUM test. Cox regression analysis and Kaplan-Meier plots were used to assess the prognostic value of LINC02560 in CRC. The correlation between the expression level of LINC02560 and the 24 immune cells in tumor microenvironment (TME) was analyzed by single sample gene set enrichment analysis (ssGSEA). Gene set enrichment analysis (GSEA) was conducted to detect potential biological processes associated with LINC02560 in CRC.

Results: LINC02560 was significantly up-regulated in CRC in comparison to normal samples. There are significant differences in the expression of LINC02560 in different subgroups of N stage, M stage, carcinoembryonic antigen (CEA) level, residual tumor, TP53 status and pathological stage. The high LINC02560 expression indicated poor overall survival (OS) and progress free interval (PFI) in patients with CRC. Moreover, the multivariate Cox analysis demonstrated that the expression of LINC02560 was an independent prognosis-predicting factor for OS in CRC patients. GSEA indicated that high expression of LINC02560 was involved in MAPK, Wnt, and PPAR signaling pathways and participated in humoral immune processes. We also identified that LINC02560 expression had a negative correlation with 4 kinds of immune cells.

Conclusions: In summary, our research results indicate that LINC02560 may be a potential prognostic biomarker. It is involved in the occurrence and development of CRC and may affect the prognosis of CRC patients by regulating immune cells in the TME.

Keywords: Colorectal cancer, LINC0256, Prognosis, Tumor immune microenvironment