B7-H3 inhibits apoptosis of gastric cancer cell by interacting with Fibronectin

Sun, Meiyun; Xie, Jinjing; Zhang, Dongze; Chen, Chunyang; Lin, Simin; Chen, Yan; Zhang, Guangbo

doi:10.7150/jca.59263



International Journal of Biological Sciences

International Journal of Medical Sciences

Theranostics

Journal of Genomics

Nanotheranostics

Global reach, higher impact

Full Text | PDF

J Cancer 2021; 12(24):7518-7526. doi:10.7150/jca.59263 This issue Cite

Research Paper

B7-H3 inhibits apoptosis of gastric cancer cell by interacting with Fibronectin

Meiyun Sun^1,2#, Jinjing Xie^1,2#, Dongze Zhang², Chunyang Chen², Simin Lin^1,2, Yan Chen^2✉, Guangbo Zhang^1,2✉

1. Medical College of Soochow University, 199 Ren ai Road, Suzhou, Jiangsu Province, 215100, China.
2. Jiangsu Institute of Clinical Immunology, The First Affiliated Hospital of Soochow University, 708 Ren min Road, Suzhou, Jiangsu Province, 215100, China.
#These authors contributed equally to this work.

Citation:

Sun M, Xie J, Zhang D, Chen C, Lin S, Chen Y, Zhang G. B7-H3 inhibits apoptosis of gastric cancer cell by interacting with Fibronectin. J Cancer 2021; 12(24):7518-7526. doi:10.7150/jca.59263. https://www.jcancer.org/v12p7518.htm

Other styles

Abstract

Anti-apoptosis has been widely accepted as a hallmark of malignancy. B7-H3, a type I transmembrane protein, plays a key role in anti-apoptosis and immune escape, but its regulation during cancer development remains unclear. To investigate how the effect of anti-apoptosis is regulated by B7-H3 in gastric cancer, we stably knocked down B7-H3 gene by shRNA in MGC-803 and MKN-45 cells. The correlation between B7-H3 and Fibronectin (FN) expression were investigated by bioinformatics in public data from TCGA (The Cancer Genome Atlas). Here, we reported that B7-H3 expression is positively correlated with FN in clinical gastric cancer samples, and B7-H3 promoted adhesion and inhibited apoptosis of gastric cancer cell through an FN-dependent pathway. Mechanistically, B7-H3 interacted with FN and subsequently activated PI3K/AKT signaling pathway, a critical mediator of oncogenic signaling. In addition, exogenous FN could inhibit the expression of pro-apoptosis-related proteins such as Caspase 3, Caspase 8, Caspase 9, Bax , p53, Apaf-1 and Cleaved PARP, and upregulated the levels of signal molecule p-PI3K, p-AKT and anti-apoptotic proteins Bcl-2 in B7-H3^high group, as compared with those in B7-H3^low group. In conclusion, we here for the first time revealed that B7-H3 inhibits apoptosis of gastric cancer cell through regulation of FN-mediated PI3K/AKT signaling pathways.

Keywords: Gastric cancer, B7-H3, Fibronectin, adhesion, apoptosis

Citation styles

APA

Sun, M., Xie, J., Zhang, D., Chen, C., Lin, S., Chen, Y., Zhang, G. (2021). B7-H3 inhibits apoptosis of gastric cancer cell by interacting with Fibronectin. Journal of Cancer, 12(24), 7518-7526. https://doi.org/10.7150/jca.59263.

ACS

Sun, M.; Xie, J.; Zhang, D.; Chen, C.; Lin, S.; Chen, Y.; Zhang, G. B7-H3 inhibits apoptosis of gastric cancer cell by interacting with Fibronectin. J. Cancer 2021, 12 (24), 7518-7526. DOI: 10.7150/jca.59263.

NLM

CSE

Sun M, Xie J, Zhang D, Chen C, Lin S, Chen Y, Zhang G. 2021. B7-H3 inhibits apoptosis of gastric cancer cell by interacting with Fibronectin. J Cancer. 12(24):7518-7526.

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.