1. Department of Hematology and Breast Cancer, Cancer Hospital of China Medical University, Shenyang 110042, Liaoning, P.R.China.
2. Department of Hematology and Breast Cancer, Liaoning Cancer Hospital & Institute, Shenyang 110042, Liaoning, P.R.China.
Background: It has been verified that long noncoding RNAs (lncRNAs) may participate in the pathogenesis of various human diseases. This study aims to investigate the roles of lncRNA SNHG5 in diffuse large B cell lymphoma (DLBC), especially the impacts of lncRNA SNHG5 on proliferation and migration of human diffuse large B cell lymphoma cells and the related mechanism.
Methods: qRT-PCR analysis was carried out to examine the expression pattern of SNHG5 in DLBC tissue and adjacent normal tissue. The effect of SNHG5 on the proliferation, apoptosis, migration, and invasion of DLBC cells was detected by MTT, flow cytometry analysis, wound healing assay and transwell assay. The correlation between SNHG5, miR-181-5p and XIAP were certified by bioinformatics analysis, and dual-luciferase reporter assay. Furthermore, rescue assays were performed to analyze the effects of SNHG5-miR-181-5p-XIAP axis on the biological behaviors of diffuse large B cell lymphoma cells. Finally, the effects of SNHG5 axis on the growth of DLBC tumor was examined by in vivo analysis.
Results: SNHG5 was significantly upregulated in diffuse large B cell lymphoma tissues. Knockdown of SNHG5 inhibited the proliferation, migration, and invasion of diffuse large B cell lymphoma cells in vitro and in vivo. LncRNA SNHG5 acted as a ceRNA through binding with miR-181-5p in DLBC cells.
Conclusion: LncRNA SNHG5 may promote proliferation and migration of diffuse large B cell lymphoma cells via targeting miR-181-5p/XIAP.
Keywords: SNHG5, miR-181-5p, diffuse large B cell lymphoma, competing endogenous RNA