MicroRNA 133A Regulates Cell Proliferation, Cell Migration, and Apoptosis in Colorectal Cancer by Suppressing CDH3 Expression

Sharma, Grinsun; Mo, Ji-Su; Lamichhane, Santosh; Chae, Soo-Cheon

doi:10.7150/jca.82916

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J Cancer 2023; 14(6):881-894. doi:10.7150/jca.82916 This issue Cite

Research Paper

MicroRNA 133A Regulates Cell Proliferation, Cell Migration, and Apoptosis in Colorectal Cancer by Suppressing CDH3 Expression

Grinsun Sharma¹, Ji-Su Mo², Santosh Lamichhane¹, Soo-Cheon Chae^1,2✉

1. Department of Pathology, School of Medicine, Wonkwang University, Iksan, Chonbuk 54538, Republic of Korea
2. Digestive Disease Research Institute, Wonkwang University, Iksan, Chonbuk 54538, Republic of Korea

Citation:

Sharma G, Mo JS, Lamichhane S, Chae SC. MicroRNA 133A Regulates Cell Proliferation, Cell Migration, and Apoptosis in Colorectal Cancer by Suppressing CDH3 Expression. J Cancer 2023; 14(6):881-894. doi:10.7150/jca.82916. https://www.jcancer.org/v14p0881.htm

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Abstract

MicroRNAs are endogenous, non-coding RNA that play an essential role in colorectal carcinoma (CRC) pathogenesis by targeting specific genes. This research aimed to determine and validate the target genes of the MIR133A associated with CRC. We verified that cadherin 3 (CDH3) is the direct target gene of MIR133A using a luciferase reporter assay, quantitative RT-PCR, and western blot analyses. CDH3 mRNA and protein expression were reduced significantly in CRC cells after transfection with MIR133A or siCDH3. We also verified that MIR133A regulated CDH3-mediated catenin, matrix metalloproteinase, apoptosis, and the epithelial-mesenchymal transition (EMT) pathway. Knockdown of CDH3 in CRC cell lines by siCDH3 produced similar results. Compared with adjacent non-tumor tissues, CDH3 protein expression was upregulated in CRC tissues, which is further confirmed by immunohistochemistry. Additionally, molecular and functional studies revealed that cell viability, migration, and colony formation were significantly reduced, and apoptosis was increased in CRC cell lines transfected with MIR133A or siCDH3. Our results suggest that MIR133A regulates CDH3 expression in human CRC.

Keywords: MIR133A, CDH3, Cell viability, Colorectal Cancer, EMT

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APA

Sharma, G., Mo, J.S., Lamichhane, S., Chae, S.C. (2023). MicroRNA 133A Regulates Cell Proliferation, Cell Migration, and Apoptosis in Colorectal Cancer by Suppressing CDH3 Expression. Journal of Cancer, 14(6), 881-894. https://doi.org/10.7150/jca.82916.

ACS

Sharma, G.; Mo, J.S.; Lamichhane, S.; Chae, S.C. MicroRNA 133A Regulates Cell Proliferation, Cell Migration, and Apoptosis in Colorectal Cancer by Suppressing CDH3 Expression. J. Cancer 2023, 14 (6), 881-894. DOI: 10.7150/jca.82916.

NLM

CSE

Sharma G, Mo JS, Lamichhane S, Chae SC. 2023. MicroRNA 133A Regulates Cell Proliferation, Cell Migration, and Apoptosis in Colorectal Cancer by Suppressing CDH3 Expression. J Cancer. 14(6):881-894.

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