The Contributions of Trace Elements on Molecular Subtype-Specific Colorectal Cancer

Bai, Dong-Xiao; Xiao, Jian-an; Huang, Tian-Chen; Shen, Zhi-Ling; Li, Lei; Ding, Fei-Fei; Wen, Ming; Wu, Shou-Xin; Liu, Xiao-Chen; Jiang, Hui-Hui

doi:10.7150/jca.81686



International Journal of Biological Sciences

International Journal of Medical Sciences

Theranostics

Journal of Genomics

Nanotheranostics

Global reach, higher impact

Full Text | PDF

J Cancer 2023; 14(9):1486-1498. doi:10.7150/jca.81686 This issue Cite

Research Paper

The Contributions of Trace Elements on Molecular Subtype-Specific Colorectal Cancer

Dong-Xiao Bai, MD¹, Jian-an Xiao, BS¹, Tian-Chen Huang, PhD¹, Zhi-Ling Shen, BS¹, Lei Li, MD¹, Fei-Fei Ding, MD², Ming Wen, MD², Shou-Xin Wu, PhD², Xiao-Chen Liu, BS^3✉, Hui-Hui Jiang, PhD^2✉

1. The Fourth Department of General Surgery, Anyang Tumor Hospital, The Affiliated Anyang Tumor Hospital of Henan University of Science and Technology, Anyang 455000, China.
2. Zhangjiang Center for Translational Medicine, Shanghai Biotecan Pharmaceuticals Co., Ltd., Shanghai 200021, China.
3. Department of General Surgery, Affiliated Three Two Zero One Hospital of Xi'an Jiaotong University, Hanzhong 723099, China.

Citation:

Bai DX, Xiao Ja, Huang TC, Shen ZL, Li L, Ding FF, Wen M, Wu SX, Liu XC, Jiang HH. The Contributions of Trace Elements on Molecular Subtype-Specific Colorectal Cancer. J Cancer 2023; 14(9):1486-1498. doi:10.7150/jca.81686. https://www.jcancer.org/v14p1486.htm

Other styles

Abstract

Purpose: Although growing studies have reported the disturbances of trace elements (TEs) homeostasis was closely associated with the occurrence of colorectal cancer (CRC), the clinical value of TEs in CRC with different molecular subtypes was largely unknown. This study aimed to explore the correlation between KRAS mutations/MSI status and serum TEs levels in patients with CRC.

Methods: The serum concentrations of 18 TEs were detected by inductively coupled plasma emission spectrometry (ICP-MS). MSI status (two mononucleotides: BAT25, BAT26, three dinucleotides: D2S123, D5S346, and D17S250), KRAS (G516T, G517A, G518C, G520T, G521A, G522C, and G532A) mutations were detected by the multiplex fluorescent PCR and the real-time fluorescent quantitative PCR, respectively. The correlations among KRAS mutations/MSI status, demographic and clinical characteristics, and TEs were analyzed by Spearman correlation analysis.

Results: The propensity score matching (PSM) analysis was adopted to minimize differences between groups. Before PSM, 204 CRC patients were recruited in this study, including 123 KRAS-negative patients and 81 KRAS-positive patients according to the test results of KRAS mutations, and 165 MSS patients and 39 MSI patients based on MSI detection. After PSM, the serum concentration of Mn was significantly lower in CRC patients with KRAS mutations than those without KRAS mutations, and a significant negative correlation was observed between Mn and Pb in the KRAS-positive cases. CRC patients carrying MSI had a significantly lower level of Rb compared to MSS patients. Importantly, Rb was significantly positively correlated with Fe, Mn, Se, and Zn in patients with MSI. Collectively, all our data indicated that the occurrence of different molecular events might be accompanied by different alterations in types and levels of serum TEs.

Conclusions: CRC patients with different molecular subtypes presented different alterations in types and levels of serum TEs. Mn was significantly negatively correlated with the KRAS mutations, and Rb was noticeably negatively correlated with the MSI status, indicating certain TEs might contribute to the pathogenesis of molecular subtype-specific colorectal cancer.

Keywords: colorectal cancer, blood, KRAS mutations, microsatellite instability, trace elements

Citation styles

APA

Bai, D.X., Xiao, J.a., Huang, T.C., Shen, Z.L., Li, L., Ding, F.F., Wen, M., Wu, S.X., Liu, X.C., Jiang, H.H. (2023). The Contributions of Trace Elements on Molecular Subtype-Specific Colorectal Cancer. Journal of Cancer, 14(9), 1486-1498. https://doi.org/10.7150/jca.81686.

ACS

Bai, D.X.; Xiao, J.a.; Huang, T.C.; Shen, Z.L.; Li, L.; Ding, F.F.; Wen, M.; Wu, S.X.; Liu, X.C.; Jiang, H.H. The Contributions of Trace Elements on Molecular Subtype-Specific Colorectal Cancer. J. Cancer 2023, 14 (9), 1486-1498. DOI: 10.7150/jca.81686.

NLM

CSE

Bai DX, Xiao Ja, Huang TC, Shen ZL, Li L, Ding FF, Wen M, Wu SX, Liu XC, Jiang HH. 2023. The Contributions of Trace Elements on Molecular Subtype-Specific Colorectal Cancer. J Cancer. 14(9):1486-1498.

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.