Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, China. *These authors contributed equally to this work.
✉ Corresponding authors: Yuehua Li, The Second Affiliated Hospital of Kunming Medical University, 374 Dianmian Road, Kunming, Yunnan 650101, China. Email: liyuehua1971com. Yang Ke, The Second Affiliated Hospital of Kunming Medical University, 374 Dianmian Road, Kunming, Yunnan 650101, China. Email: keyangedu.cn.More
Citation:
Kang Q, Zheng K, Jiang GM, Li YK, Liang YB, Geng Q, Qian CH, Wang QB, He ZY, Huang SQ, Yang C, Li J, Li YH, Ke Y. Brucine suppresses proliferation and promotes apoptosis of human cholangiacarcinoma cells via the inhibition of COX2 expression. J Cancer 2023; 14(14):2700-2706. doi:10.7150/jca.87514. https://www.jcancer.org/v14p2700.htm
Aims The aim of this study was to investigate the anti-tumor efficacy of brucine on intrahepatic cholangiocarcinoma (ICC).
Methods ICC QBC939 cells were treated with brucine, cell viability, cell cycle and apoptosis were analyzed using CCK-8 and flow cytometry. The expression of COX-2 and apoptosis related proteins Casp3, Bax and Bcl-2 were detected by Western blot analysis. QBC939 cells were subcutaneously transplanted into nude mice and the mice were injected with brucine intraperitoneally. The expression of Ki67, COX-2 and apoptosis related proteins were detected by immunohistochemical staining and Western blot analysis.
Results Brucine significantly inhibited the proliferation and cell cycle progression while promoted the apoptosis of QBC939 cells. The expression of the apoptotic proteins Casp3 and Bax was upregulated, while the expression of Bcl-2 and COX-2 was downregulated in QBC939 cells with brucine treatment. Moreover, the overexpression of COX-2 could antagonize the effects of brucine on QBC939 cells. In vivo, brucine inhibited subcutaneous tumor formation in nude mice, and the expression of Ki67, COX-2 and Bcl-2 decreased while the expression of Casp3 and Bax increased in tumor tissues from nude mice with brucine treatment.
Conclusions Brucine can significantly inhibit the progression of cholangiocarcinoma in vitro and in vivo, and the mechanism may be related to the inhibition of COX-2 expression.
Kang, Q., Zheng, K., Jiang, G.M., Li, Y.K., Liang, Y.B., Geng, Q., Qian, C.H., Wang, Q.B., He, Z.Y., Huang, S.Q., Yang, C., Li, J., Li, Y.H., Ke, Y. (2023). Brucine suppresses proliferation and promotes apoptosis of human cholangiacarcinoma cells via the inhibition of COX2 expression. Journal of Cancer, 14(14), 2700-2706. https://doi.org/10.7150/jca.87514.
ACS
Kang, Q.; Zheng, K.; Jiang, G.M.; Li, Y.K.; Liang, Y.B.; Geng, Q.; Qian, C.H.; Wang, Q.B.; He, Z.Y.; Huang, S.Q.; Yang, C.; Li, J.; Li, Y.H.; Ke, Y. Brucine suppresses proliferation and promotes apoptosis of human cholangiacarcinoma cells via the inhibition of COX2 expression. J. Cancer 2023, 14 (14), 2700-2706. DOI: 10.7150/jca.87514.
NLM
Kang Q, Zheng K, Jiang GM, Li YK, Liang YB, Geng Q, Qian CH, Wang QB, He ZY, Huang SQ, Yang C, Li J, Li YH, Ke Y. Brucine suppresses proliferation and promotes apoptosis of human cholangiacarcinoma cells via the inhibition of COX2 expression. J Cancer 2023; 14(14):2700-2706. doi:10.7150/jca.87514. https://www.jcancer.org/v14p2700.htm
CSE
Kang Q, Zheng K, Jiang GM, Li YK, Liang YB, Geng Q, Qian CH, Wang QB, He ZY, Huang SQ, Yang C, Li J, Li YH, Ke Y. 2023. Brucine suppresses proliferation and promotes apoptosis of human cholangiacarcinoma cells via the inhibition of COX2 expression. J Cancer. 14(14):2700-2706.
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