1. Department of Hematology, Jagiellonian University Medical College, Krakow, Poland
2. Department of Transplantation, Jagiellonian University Medical College, Krakow, Poland
3. Department of Infectious Diseases, Jagiellonian University Medical College, Krakow, Poland
4. Department of Radioligands, Faculty of Pharmacy, Jagiellonian University Medical College, Krakow, Poland
5. Department of Medicine, John Dempsey Hospital, University of Connecticut, Farmington, CT, USA
The bone marrow microenvironment plays a key role in the stimulation of growth and survival of multiple myeloma (MM) cells. We investigated whether membrane microfragments (MFBs) exert a stimulatory effect on mesenchymal stem cell (MSC) gene expression or differentiation. MSCs from patients with multiple myeloma (MMBM-MSCs) proliferated at a slower rate than MSCs from healthy volunteers (BM-MSCs), and fewer MMBM-MSCs adhered to the substrate as compared to BM-MSCs. Phenotypic analysis revealed that MMBM-MSCs and BM-MSCs differed significantly in terms of their CD166 and CXCR4 expressions. In conclusion, our comparative analysis of mesenchymal cells from MM patients and healthy volunteers revealed differences in the genetic and phenotypic profiles of these two populations, their potential for osteodifferentiation, and expression of surface antigens. Moreover, we showed that membrane MFBs may alter the genetic profile of MSCs, leading to disorders of their osteodifferentiation, and interact with the WNT pathway via presentation of the DKK-1 protein.
Keywords: bone marrow, genotype, membrane microfragments, microenvironment, multiple myeloma, osteodifferentiation