J Cancer 2017; 8(16):3371-3377. doi:10.7150/jca.20983 This issue Cite

Research Paper

Phosphorylated Protein Kinase C (Zeta/Lambda) Expression in Colorectal Adenocarcinoma and Its Correlation with Clinicopathologic Characteristics and Prognosis

Min-kyung Yeo1, Ji Yeon Kim3, In-ock Seong1, Jin-Man Kim2✉, Kyung-Hee Kim1✉

1. Department of Pathology, Cancer Research Institute, Chungnam National University School of Medicine, Daejeon, Republic of Korea;
2. Department of Pathology and Medical Science, Chungnam National University School of Medicine, Daejeon, Republic of Korea;
3. Department of Surgery, Chungnam National University Hospital, Daejeon, Republic of Korea.

Citation:
Yeo Mk, Kim JY, Seong Io, Kim JM, Kim KH. Phosphorylated Protein Kinase C (Zeta/Lambda) Expression in Colorectal Adenocarcinoma and Its Correlation with Clinicopathologic Characteristics and Prognosis. J Cancer 2017; 8(16):3371-3377. doi:10.7150/jca.20983. https://www.jcancer.org/v08p3371.htm
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Abstract

Background: Protein kinase C zeta/lambda (PKCζ/λ) is a family of protein kinase enzymes that contributes to cell proliferation and regulation, which are important for cancer development. PKCζ/λ has been shown to be an important regulator of tumorigenesis in intestinal cancer. The phosphorylated form of PKCζ/λ, p-PKCζ/λ, is suggested as an active form of PKCζ/λ. However, p-PKCζ/λ expression and its clinicopathologic implication in colorectal adenocarcinoma (CRAC) are unclear.

Methods: Seven whole-tissue sections of malignant polyps containing both non-neoplastic and neoplastic mucosa, 11 adenomas with low-grade dysplasia, and 173 CRACs were examined by immunohistochemistry and western blot assay for p-PKCζ/λ protein expression. The association of p-PKCζ/λ expression with clinicopathologic factors including patient survival was studied.

Results: In non-neoplastic epithelia, p-PKCζ/λ showed a weak cytoplasmic immunostaining. Adenomas and CRACs demonstrated up-regulated p-PKCζ/λ detection. Cytoplasmic p-PKCζ/λ expression was higher in CRAC than in adenoma. In CRACs, p-PKCζ/λ expression was inversely correlated with pathologic TNM stage (I-II versus III-IV) and poor differentiation. Statistical correlations between low expression of p-PKCζ/λ with shortened overall survival and disease-free survival were seen (p=0.004 and p=0.034, respectively).

Conclusions: P-PKCζ/λ overexpression is implicated in tumorigenesis but down-regulation was a poor prognostic factor in CRAC.

Keywords: Protein kinase C zeta, protein kinase C lambda, colorectal adenocarcinoma.


Citation styles

APA
Yeo, M.k., Kim, J.Y., Seong, I.o., Kim, J.M., Kim, K.H. (2017). Phosphorylated Protein Kinase C (Zeta/Lambda) Expression in Colorectal Adenocarcinoma and Its Correlation with Clinicopathologic Characteristics and Prognosis. Journal of Cancer, 8(16), 3371-3377. https://doi.org/10.7150/jca.20983.

ACS
Yeo, M.k.; Kim, J.Y.; Seong, I.o.; Kim, J.M.; Kim, K.H. Phosphorylated Protein Kinase C (Zeta/Lambda) Expression in Colorectal Adenocarcinoma and Its Correlation with Clinicopathologic Characteristics and Prognosis. J. Cancer 2017, 8 (16), 3371-3377. DOI: 10.7150/jca.20983.

NLM
Yeo Mk, Kim JY, Seong Io, Kim JM, Kim KH. Phosphorylated Protein Kinase C (Zeta/Lambda) Expression in Colorectal Adenocarcinoma and Its Correlation with Clinicopathologic Characteristics and Prognosis. J Cancer 2017; 8(16):3371-3377. doi:10.7150/jca.20983. https://www.jcancer.org/v08p3371.htm

CSE
Yeo Mk, Kim JY, Seong Io, Kim JM, Kim KH. 2017. Phosphorylated Protein Kinase C (Zeta/Lambda) Expression in Colorectal Adenocarcinoma and Its Correlation with Clinicopathologic Characteristics and Prognosis. J Cancer. 8(16):3371-3377.

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