1. Department of Vascular Surgery, the First Hospital of Jilin University, Changchun, 130021, P.R. China;
2. Department of Clinical Medicine, Norman Bethune Health Science Center of Jilin University, Changchun, 130021,P.R. China;
3. Department of Pediatric Emergency, the First Hospital of Jilin University, Changchun, 130021, P.R. China;
4. Department of Otolaryngology Head and Neck surgery, the First Hospital of Jilin University, Changchun, 130021, P.R. China;
5. Department of Cell Biology, College of Life Science, Zhejiang Chinese Medical University, Hangzhou, 310053, China;
6. Department of Regenerative Medicine, School of Pharmaceutical Science, Jilin University, Changchun, 130021, P.R. China.
Glioma is a worldwide malignancy, which displays significantly active metastasis and angiogenesis. Interaction between long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) has been shown to play crucial role in regulating tumor properties. However, the potential of lncRNA X-inactive specific transcript (XIST) to function as a miRNA regulator and its relevance in glioma tumorigenicity and angiogenesis have so far remained unclear. Expression analysis of lncRNA XIST in glioma cells revealed its significant up-regulation. Interestingly, silencing of XIST repressed both metastatic and pro-angiogenic ability in vitro as well as in vivo. Subsequent studies revealed that lncRNA XIST expression inversely correlated with miR-429 expression in glioma cells; miR-429 modulated XIST expression by directly targeting the XIST gene sequence. In addition, miR-429 inhibitor restored metastatic and pro-angiogenic ability of gliomas abolished by silencing XIST. Our data provide insight into the key roles of the lncRNA-miRNA functional network in gliomas, which can aid in developing new therapeutic strategies for gliomas through clinical trials.
Keywords: lncRNA XIST, miR-429, glioma, HBMVECs, angiogenesis