Tripartite motif-containing 15 overexpression in non-small cell lung cancer is associated with poor patient prognoses

Han, Xiaoying; Huang, Cuicui; Qu, Xiao; Liu, Shaorui; Yang, Xudong; Wang, Yu; Bie, Fenglong; Liu, Qi; Du, Jiajun

doi:10.7150/jca.27856

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J Cancer 2019; 10(4):843-852. doi:10.7150/jca.27856 This issue Cite

Research Paper

Tripartite motif-containing 15 overexpression in non-small cell lung cancer is associated with poor patient prognoses

Xiaoying Han^1,2*, Cuicui Huang^1*, Xiao Qu¹, Shaorui Liu¹, Xudong Yang¹, Yu Wang¹, Fenglong Bie¹, Qi Liu¹, Jiajun Du^1,3✉

1. The Institute of Oncology, Shandong Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, Jinan, 250021 P.R. China
2. Department of Oncology, Shandong Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, Jinan, 250021 P.R. China
3. Department of Thoracic Surgery, Shandong Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, Jinan, 250021 P.R. China
*These authors contributed equally to this work.

Citation:

Han X, Huang C, Qu X, Liu S, Yang X, Wang Y, Bie F, Liu Q, Du J. Tripartite motif-containing 15 overexpression in non-small cell lung cancer is associated with poor patient prognoses. J Cancer 2019; 10(4):843-852. doi:10.7150/jca.27856. https://www.jcancer.org/v10p0843.htm

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Abstract

Purpose: This study aimed to comprehensively investigate the differential expression and prognostic indicators of the tripartite motif-containing (TRIM) gene family in non-small cell lung cancer (NSCLC).

Methods: The Cancer Genome Atlas (TCGA) Research Network and three datasets from Gene Expression Omnibus (GEO) database were used to assess TRIM gene family expression patterns in NSCLC. Quantitative real-time PCR and immunohistochemistry (IHC) were conducted to confirm differentially expressed genes (DEGs). Kaplan-Meier survival analysis and univariate Cox regression analysis were carried out to analyze the association between TRIM gene expression and NSCLC prognoses. Gene set enrichment analysis (GSEA) was carried on for the predict the biological processes.

Results: Of the 78 TRIM family members measured, TRIM15 was selected due to the DEGs and the prognostic value regarding NSCLC. In lung squamous cell carcinoma (LUSC), the Log₂ fold change (Log₂FC) of TRIM15 was 5.16 (p= 0.00575), whereas in lung adenocarcinoma (LUAD), it was 6.37 (p =6.78E-07). TRIM15 upregulation was related to poor prognoses in both LUSC (HR 1.353; 95%CI 1.023-1.789; p =0.034) and LUAD (HR 1.560; 95%CI 1.159-2.101; p =0.003). Using immunohistochemistry, TRIM15 expression was significantly higher in NSCLC tissues compared with that of matched normal tissues (p =0.0009), and similar findings were generated with tissue microarray analysis (p<0.0001).

Conclusion: TRIM15 could act as a diagnostic predictor or therapeutic target for lung cancer treatments.

Keywords: Non-small cell lung cancer, E3 ubiquitin ligases, survival analysis, TRIM family genes, TRIM15

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APA

Han, X., Huang, C., Qu, X., Liu, S., Yang, X., Wang, Y., Bie, F., Liu, Q., Du, J. (2019). Tripartite motif-containing 15 overexpression in non-small cell lung cancer is associated with poor patient prognoses. Journal of Cancer, 10(4), 843-852. https://doi.org/10.7150/jca.27856.

ACS

Han, X.; Huang, C.; Qu, X.; Liu, S.; Yang, X.; Wang, Y.; Bie, F.; Liu, Q.; Du, J. Tripartite motif-containing 15 overexpression in non-small cell lung cancer is associated with poor patient prognoses. J. Cancer 2019, 10 (4), 843-852. DOI: 10.7150/jca.27856.

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CSE

Han X, Huang C, Qu X, Liu S, Yang X, Wang Y, Bie F, Liu Q, Du J. 2019. Tripartite motif-containing 15 overexpression in non-small cell lung cancer is associated with poor patient prognoses. J Cancer. 10(4):843-852.

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