J Cancer 2019; 10(17):4000-4008. doi:10.7150/jca.29029 This issue

Research Paper

Knockdown of LncRNA RHPN1-AS1 Inhibits Cell Migration, Invasion and Proliferation in Head and Neck Squamous Cell Carcinoma

Xiaowen Qiu1,2, Zhuo Lei3, Zeyou Wang4, Yuming Xu1, Chang Liu1, Panchun Li1, Hanjiang Wu1, Zhaojian Gong1✉

1. Department of Stomatology, Second Xiangya Hospital, Central South University, Changsha 410011, Hunan, China;
2. Department of Stomatology, Haikou people's Hospital, Haikou 570208, Hainan, China;
3. Xiangya Stomatological Hospital & School of Stomatology, Central South University, Changsha 410008, Hunan, China;
4. Department of Laboratory Medicine, Second Xiangya Hospital, Central South University, Changsha 410011, Hunan, China.

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Citation:
Qiu X, Lei Z, Wang Z, Xu Y, Liu C, Li P, Wu H, Gong Z. Knockdown of LncRNA RHPN1-AS1 Inhibits Cell Migration, Invasion and Proliferation in Head and Neck Squamous Cell Carcinoma. J Cancer 2019; 10(17):4000-4008. doi:10.7150/jca.29029. Available from https://www.jcancer.org/v10p4000.htm

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Abstract

Previous studies have revealed that long non-coding RNAs (lncRNAs) are involved in head and neck squamous cell carcinoma (HNSCC) progression. However, the detailed roles of lncRNA RHPN1-AS1 remain to be elucidated. In this study, by analyzing online RNA-Seq data, we found that RHPN1-AS1 was upregulated in HNSCC tissues and that its expression level was associated with neoplasm histologic grade. High expression of RHPN1-AS1 was also confirmed in HNSCC tissues. Knockdown of RHPN1-AS1 inhibited tumor cell migration, invasion and proliferation in HNSCC. Furthermore, inhibition of RHPN1-AS1 suppressed the expression of epithelial-mesenchymal transition (EMT)-related genes (β-Catenin, Claudin-1 and Vimentin) in HNSCC cells. Collectively, our results suggest that RHPN1-AS1, acting as an oncogene, may be a potential diagnostic and therapeutic target in HNSCC.

Keywords: Long non-coding RNA, RHPN1-AS1, HNSCC, EMT