J Cancer 2020; 11(5):1212-1222. doi:10.7150/jca.38283 This issue

Research Paper

Prognostic value of downregulated 5-hydroxymethylcytosine expression in renal cell carcinoma: a 10 year follow-up retrospective study

Song Chen1*, Qiang Zhou1*, Tongzu Liu1, Weibing Zhang1, Xian-Tao Zeng1,2✉, Zhongqiang Guo1✉

1. Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.
2. Center for Evidence-Based and Translational Medicine, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.
*These authors contributed equally to this work.

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Citation:
Chen S, Zhou Q, Liu T, Zhang W, Zeng XT, Guo Z. Prognostic value of downregulated 5-hydroxymethylcytosine expression in renal cell carcinoma: a 10 year follow-up retrospective study. J Cancer 2020; 11(5):1212-1222. doi:10.7150/jca.38283. Available from https://www.jcancer.org/v11p1212.htm

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Abstract

Graphic abstract

5-hydroxymethylcytosine (5hmC) is converted from DNA methylation of cytosine (5mC) by the catalysis of TET proteins, and proposed to be involved in tumorigenesis. However, the prognostic value of 5hmC in renal cell carcinoma (RCC) is still unclear. This study aimed to define the clinical significance of 5hmC in RCC. We performed dot blot assays to measure the relative expression of 5hmC in RCC. We reviewed the clinical records of 310 RCC patients and performed immunohistochemical (IHC) staining of 5hmC. The overall survival (OS) and cancer specific survival (CSS) of all patients were recorded over a 10-year follow-up period. Effective prognostic nomograms which contained 5hmC were established to provide individualized OS and CSS in RCC. 5hmC expression level was significantly decreased in RCC tissues compared with those in the normal counterparts. Kaplan-Meier curves revealed that high 5hmC expression had a good prognostic impact on RCC patients. Cox multivariate survival analyses further indicated 5hmC was an independent prognostic factor for RCC survival. Nomograms constructed based on cox regression analysis were available to calculate the survival probability directly. Calibration curves displayed good agreements. The findings were validated with an independent external cohort included 77 RCC cases. Thus, we believe we have found a significative prognostic factor for RCC.

Keywords: renal cell carcinoma, 5-hydroxymethylcytosine, clinical significance, nomogram.