J Cancer 2020; 11(9):2716-2723. doi:10.7150/jca.34902 This issue

Research Paper

MicroRNA-145 suppresses epithelial to mesenchymal transition in pancreatic cancer cells by inhibiting TGF-β signaling pathway

Shaojun Chen1*, Junyi Xu2*, Yao Su3*, Li Hua1, Chengjun Feng1, Zhan Lin4, Haixin Huang1✉, Yongqiang Li5✉

1. Department of Oncology, the Forth Affiliated Hospital of Guangxi Medical University, Liuzhou, Guangxi, China
2. Department of general surgery, The Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, Guangxi, China.
3. Nanjing Zhongshan Biomedical Translational Institute, Nanjing, Jiangsu, China
4. Department of Oncology, The Yulin First People's Hospital , Yulin, Guangxi ,China
5. Department of Chemotherapy, the Affiliated Tumor Hospital of Guangxi Medical University, Nanning, Guangxi, China
*Shaojun Chen, Junyi Xu and Yao Su contributed equally to this work

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Citation:
Chen S, Xu J, Su Y, Hua L, Feng C, Lin Z, Huang H, Li Y. MicroRNA-145 suppresses epithelial to mesenchymal transition in pancreatic cancer cells by inhibiting TGF-β signaling pathway. J Cancer 2020; 11(9):2716-2723. doi:10.7150/jca.34902. Available from https://www.jcancer.org/v11p2716.htm

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Abstract

TGF-β signaling plays a critical role in tumor progression and many approaches have been made to inhibit its functions. MicroRNA is one of the approaches that inhibit TGF-β signaling and can be used as a promising treatment for cancer. This study explored the role of miRNA-145 in pancreatic cancer (PC) development. The expression of miRNA-145 in PC tissues and paired adjacent normal tissues was examined by qRT-PCR. The expression of miRNA-145 in PC cells and the ability of cell migration and invasion were detected both in vivo and in vitro. The results showed that miRNA-145 was down-regulated in PC tissues and PC cells. Increasing the expression of miRNA-145 in PC cells inhibited the TGF-β signaling pathway and epithelial-mesenchymal transition (EMT) process. Scratch assay and transwell assay showed that miRNA-145 inhibited the migration and invasion in PC cells. In vivo experiments confirmed that miRNA-145 mimics delayed the growth of PC xenografts comparing with miRNA-145 inhibitor. Our results suggested that miRNA-145 can inhibit epithelial to mesenchymal transition (EMT) and tumor growth by suppressing TGF-β signaling pathway. Thus, miRNA-145 could be a potential therapeutic for targeting TGF-β signaling in PC treatment.

Keywords: miRNA-145, pancreatic cancer, EMT, metastasis, TGF- β signaling