J Cancer 2020; 11(11):3199-3206. doi:10.7150/jca.33273 This issue

Research Paper

miR-204-5p Suppress Lymph Node Metastasis via Regulating CXCL12 and CXCR4 in Gastric Cancer

Juan Zhang1#, Ling Xing2#, Hongwei Xu4, Kaixuan Wang3, Junjun She5, Feiyu Shi5, Hongyu Wu3, Yongjie Sun6, Jun Gao3,6✉, Shuixiang He1✉

1. Department of Gastroenterology, The First Affiliated Hospital of Xi'an JiaoTong University, 277 Yanta West Road, Xi'an, Shaanxi, China.
2. Department of Gastroenterology, Eastern Hepatobiliary Hospital, Second Military Medical University, Shanghai, China.
3. Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai, China.
4. Department of Gastroenterology, Kunshan traditional Chinese medicine hospital. Jiangsu, China.
5. Department of General Surgery, The First Affiliated Hospital of Xi'an Jiao Tong University, 277 Yanta West Road, Xi'an, Shaanxi, China.
6. Institute of Oncology, Second Affiliated Hospital, Xi'an Medical College, China.
#These authors contributed equally to this work.

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Citation:
Zhang J, Xing L, Xu H, Wang K, She J, Shi F, Wu H, Sun Y, Gao J, He S. miR-204-5p Suppress Lymph Node Metastasis via Regulating CXCL12 and CXCR4 in Gastric Cancer. J Cancer 2020; 11(11):3199-3206. doi:10.7150/jca.33273. Available from https://www.jcancer.org/v11p3199.htm

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Abstract

Gastric cancer (GC) exhibits a poor prognosis due to extensive invasion and lymphatic metastasis in the advanced stage. In this study, we firstly found that the expression of miR-204-5p markedly decreased in GC patients' tissue and serum, especially in GC with lymphatic metastasis. And ROC analysis showed miR-204-5p also served as a predicted factor for the lymphatic metastasis of GC. CXCL12 and CXCR4 were predicted and confirmed as the functional targets of miR-204-5p by Targetscan analysis, dual luciferase assay and western blotting analysis. In addition, we further determined that miR-204-5p suppresses migration and invasion in GC. This finding elucidates new functions and mechanisms for miR-204-5p in GC development and provides a new potential diagnostic marker and therapeutic targets for GC.

Keywords: miR-204-5p, CXCR4, CXCL12, gastric cancer, migration, invasion, lymph node metastasis