J Cancer 2020; 11(23):6960-6969. doi:10.7150/jca.49117 This issue

Research Paper

Comprehensive analysis of gene regulation network and immune signatures of prognostic biomarker YAP1 in pancreatic cancer

Yuan Yang1,2,3#, Ya Zheng2,3#, Xin Liu4#, Rui Ji2,3, Zhaofeng Chen2,3, Qinghong Guo2,3, Guozhi Wu1,2,3, Yuping Wang2,3✉, Yongning Zhou2,3✉

1. The First Clinical Medical School, Lanzhou University, Lanzhou, 730000, PR China.
2. Department of Gastroenterology, The First Hospital of Lanzhou University, Lanzhou 730000, PR China.
3. Key Laboratory for Gastrointestinal Diseases of Gansu Province, The First Hospital of Lanzhou University, Lanzhou 730000, PR China.
4. Department of Gastroenterology, The 940th Hospital of Joint Logistic Support Force of PLA, Lanzhou 730050, China.
#These authors contributed equally to this article.

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Citation:
Yang Y, Zheng Y, Liu X, Ji R, Chen Z, Guo Q, Wu G, Wang Y, Zhou Y. Comprehensive analysis of gene regulation network and immune signatures of prognostic biomarker YAP1 in pancreatic cancer. J Cancer 2020; 11(23):6960-6969. doi:10.7150/jca.49117. Available from https://www.jcancer.org/v11p6960.htm

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Abstract

Background: Pancreatic cancer (PC) is one of the most common digestive malignancy, with severe cancer-related death and disease burden. Yes-associated protein 1 (YAP1) has been reported to be involved in the tumorigenesis and progression of several cancers, thus leading to poor prognosis of patients. However, the relationship between YAP1 and immune microenvironment in PC deserve more scrutiny.

Methods: GEPIA, OncoLnc, PROGgeneV2 and HPA database were utilized to analyze the expression (transcriptome and protein levels) and overall survival of YAP1 in PC. Then, we evaluated the risk factors associated with overall survival based on public data from TCGA-PAAD via Cox regression. Besides, LinkedOmics was utilized to identify co-expression genes and the potential regulation network of YAP1. Furthermore, we explored the relationship between YAP1 and immune infiltration using CIBERSORT algorithm and GEPIA database.

Results: The age, lymph node metastasis status and up-regulated YAP1 expression have been proved to be independent prognostic factors for poor prognosis. The functions of YAP1 and co-expression genes were mainly involved in the angiogenesis, immune response-regulating signaling pathway, regulation of actin cytoskeleton, NOD-like receptor signaling pathway and cytokine-cytokine receptor interaction. Specifically, increased YAP1 expression was significantly correlated with immune infiltrating levels of resting CD4+T cells.

Conclusions: Our findings provide evidence of the immune regulatory role of YAP1 in PC and help elucidate the role of YAP1 in carcinogenesis as well.

Keywords: pancreatic cancer, YAP1, immune infiltration