J Cancer 2021; 12(17):5249-5259. doi:10.7150/jca.52088 This issue

Research Paper

Antiproliferative, Apoptotic Effects and Suppression of Oxidative Stress of Quercetin against Induced Toxicity in Lung Cancer Cells of Rats: In vitro and In vivo Study

Zhaorigetu1, Islam M Farrag2, Amany Belal3, Manal H. Al Badawi4, Alsayed A. Abdelhady4, Fawzy M. A. Abou Galala5, Abdou El-Sharkawy5, Asmaa A. EL-Dahshan6, Ahmed B. M. Mehany7✉

1. Thoracic Surgery, Inner Mongolia People's Hospital, Hohhot City, Inner Mongolia Autonomous Region, 010020, China.
2. Forensic Medicine & Clinical Toxicology Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt.
3. Department of Pharmaceutical Chemistry, College of Pharmacy, Taif University, P.O. Box 11099,Taif 21944, Saudi Arabia.
4. Department of anatomy, Faculty of Medicine, Helwan University, Helwan, Egypt.
5. Department of anatomy, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.
6. Department of zoology Faculty of science (Girls branch), Al-Azhar University, Cairo, Egypt.
7. Department of zoology Faculty of science, Al-Azhar University, Cairo, Egypt.

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Citation:
Zhaorigetu , Farrag IM, Belal A, Badawi MHA, Abdelhady AA, Galala FMAA, El-Sharkawy A, EL-Dahshan AA, Mehany ABM. Antiproliferative, Apoptotic Effects and Suppression of Oxidative Stress of Quercetin against Induced Toxicity in Lung Cancer Cells of Rats: In vitro and In vivo Study. J Cancer 2021; 12(17):5249-5259. doi:10.7150/jca.52088. Available from https://www.jcancer.org/v12p5249.htm

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Abstract

Graphic abstract

In the present study, quercetin was examined against lung human cancer cells using A549 and H69 cancer cell lines in addition to normal non cancer cells (W138). Two genes Bax and Bcl-2 that play an important role in apoptosis pathways were investigated. Also Immunohistochemical study for caspase-3 which is considered as indicator for apoptosis was performed. Quercetin showed good anti proliferative activity against tested lung cancer cell lines, IC50 values on A549 are 8.65, 7.96 and 5.14 µg/ml at 24, 48 and 72h respectively. Also significant effects of quercetin on Bax, Bcl-2 and caspase-3 were observed, that can prove its ability to induce apoptosis. On the other hand quercetin showed good therapeutic effects against cyclophosphamide induced lung toxicity that were observed in the histopathology study. In vitro studies were also performed such as cell cycle analysis through flowcytometry. The obtained results from all these performed analysis proved that quercetin can induce apoptosis in human lung cancer cells, additionally quercetin showed ability to reduce MDA and increase SOD and GSHP levels which indicates its ability in suppressing oxidative stress, Quercetin has played a therapeutic role in cyclophosphamide induced lung toxicity as it has improved restoring of the damaged lung tissue as discussed in this research work.

Keywords: lung cancer, quercetin, cyclophosphamide, cell cycle, molecular docking, antioxidant enzymes