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J Cancer 2022; 13(11):3258-3267. doi:10.7150/jca.76663 This issue Cite

Research Paper

Peroxiredoxin 5 protects HepG2 cells from ethyl β-carboline-3-carboxylate-induced cell death via ROS-dependent MAPK signalling pathways

Dan-Ping Xie^1,#, Yi-Xi Gong^2,3,#, Jaihyung Lee^4,#, Eui Man Jeong^2,3, Chen-Xi Ren¹, Xiao-Yu Guo¹, Ying-Hao Han¹, Yu-Dong Cui¹, Seung-Jae Lee^5,6✉, Taeho Kwon^7,8✉, Hu-Nan Sun^1✉

1. College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, P.R. China
2. Jeju Research Institute of Pharmaceutical Sciences, College of Pharmacy, Jeju National University, Jeju, 63243, Republic of Korea
3. Interdisciplinary Graduate Program in Advanced Convergence Technology and Science, Bio-Health Materials Core-Facility Center and Practical Translational Research Center, Jeju National University, Jeju, 63243, Republic of Korea
4. Epigenetics Drug Discovery Center, Hwalmyeong Convalescence Hospital, Gapyeong, Gyeonggi 12458, Republic of Korea
5. Functional Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si, Jeonbuk 56212, Republic of Korea
6. Department of Applied Biological Engineering, Biotechnology of KRIBB School, University of Science and Technology, Daejeon 34113, Republic of Korea
7. Primate Resources Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup-si, Jeonbuk, 56216, Republic of Korea
8. Department of Functional Genomics, Bioscience of KRIBB School, University of Science and Technology, Daejeon 34113, Republic of Korea
#These authors contributed equally to this work.

✉ Corresponding authors: Taeho Kwon, Ph.D., Primate Resources Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 351-33 Neongme-gil, Ibam-myeon, Jeongeup-si, Jeonbuk, 56216, Republic of Korea. Telephone: 82-63-570-5316, Fax: 82-63-570-5309, E-mail: kwonre.kr; Seung-Jae Lee, Ph.D., Immunoregulatory Materials Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB). Telephone: 82- 63-570-5267, Fax: 82-63-570-5309, E-mail: seung99re.kr; Hu-Nan Sun, College of Life Science & Technology, Heilongjiang Bayi Agricultural University, Xinyanglu, 163319, Daqing, P. R. China. Telephone: +86 4596819300, Fax: +86 4596819295, E-mail: sunhunan76com. More

Abstract

Peroxiredoxin 5 (PRDX5) is the member of Prxs family, widely reported to be involved in various types of cell death. We previously found that PRDX5 knockdown increases the susceptibility of cell death upon oxidative stress treatment. Ethyl β-carboline-3-carboxylate (β-CCE), an alkaloid extracted from Picrasma quassioides, has been reported to play a role in neuronal disease, but its anti-cancer potential on liver cancers remains unknown. Here, we studied the effect of PRDX5 on ethyl β-carboline-3-carboxylate (β-CCE)-induced apoptosis of hepatomas. High expression level of PRDX5 was deeply related with the postoperative survival of patients with liver cancer, indicating that PRDX5 may be a biomarker of live cancer processing. Moreover, PRDX5 over-expression in HepG2 cells significantly inhibited β-CCE-induced cell apoptosis and cellular ROS levels as well as mitochondrial dysfunction. Signalling pathway analysis showed that β-CCE could significantly up-regulate the ROS-dependent MAPK signalling, which were in turn boosts the mitochondria-dependent cell apoptosis. Moreover, PRDX5 over-expression could reverse the anti-cancer effects induced by β-CCE in HepG2 cells. Our findings suggest that PRDX5 has a protective role on β-CCE-induced liver cancer cell death and provides new insights for using its anti-cancer properties for liver cancer treatment.

Keywords: Peroxiredoxin5, ethyl β-carboline-3-carboxylate, ROS, mitochondria, HepG2 cells, liver cancer

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