J Cancer 2023; 14(5):707-716. doi:10.7150/jca.81050 This issue Cite

Research Paper

Rapid microRNA detection method based on DNA strand displacement for ovarian cancer cells

Gege Sun1,*, Congzhou Chen2,*, Xin Li1,✉, Shangwei Hong3, Chuanqi Gu1, Xiaolong Shi3

1. Department of Gynecology 2, Renmin Hospital of Wuhan University, 430072, Wuhan, China
2. School of Computer Science, Beijing University of Technology, 100124, Beijing, China
3. Institute of Computing Science & Technology, Guangzhou University, 510006, Guangzhou, China
* These authors contributed equally to this work

Citation:
Sun G, Chen C, Li X, Hong S, Gu C, Shi X. Rapid microRNA detection method based on DNA strand displacement for ovarian cancer cells. J Cancer 2023; 14(5):707-716. doi:10.7150/jca.81050. https://www.jcancer.org/v14p0707.htm
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Abstract

Graphic abstract

The current cancer detection methods are heavily dependent on the component analysis of corresponding cancer antigens. There is a lack of effective and simple clinical methods of ovarian cancer screening, which hinders the early identification for ovarian cancer and its treatment. To develop a simple and rapid method for quantitative analysis of ovarian cancer, we developed a DNA strand displacement-based method and finished the rapid detection of miR-21 in ovarian cancer cells within 5 min by a one-step isothermal reaction. The fluorescence intensity trajectory had a good linear relationship with miR-21 concentrations in the range of 100 fM-100 nM, with a lower limit of 6.05 pM. This detection method is simple, faster, and accurate. Besides, it can be applied to detect the miRNA biomarkers of other cancers by changing the preset sequences of toehold.

Keywords: ovarian cancer, miR-21 detection, DNA isothermal strand displacement reaction


Citation styles

APA
Sun, G., Chen, C., Li, X., Hong, S., Gu, C., Shi, X. (2023). Rapid microRNA detection method based on DNA strand displacement for ovarian cancer cells. Journal of Cancer, 14(5), 707-716. https://doi.org/10.7150/jca.81050.

ACS
Sun, G.; Chen, C.; Li, X.; Hong, S.; Gu, C.; Shi, X. Rapid microRNA detection method based on DNA strand displacement for ovarian cancer cells. J. Cancer 2023, 14 (5), 707-716. DOI: 10.7150/jca.81050.

NLM
Sun G, Chen C, Li X, Hong S, Gu C, Shi X. Rapid microRNA detection method based on DNA strand displacement for ovarian cancer cells. J Cancer 2023; 14(5):707-716. doi:10.7150/jca.81050. https://www.jcancer.org/v14p0707.htm

CSE
Sun G, Chen C, Li X, Hong S, Gu C, Shi X. 2023. Rapid microRNA detection method based on DNA strand displacement for ovarian cancer cells. J Cancer. 14(5):707-716.

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