J Cancer 2019; 10(23):5883-5890. doi:10.7150/jca.32267 This issue Cite

Research Paper

The transcriptome difference between colorectal tumor and normal tissues revealed by single-cell sequencing

Guo-Liang Zhang1, Le-Lin Pan1, Tao Huang2✉, Jin-Hai Wang1✉

1. Department of Colorectal Surgery, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang, China
2. Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China

Citation:
Zhang GL, Pan LL, Huang T, Wang JH. The transcriptome difference between colorectal tumor and normal tissues revealed by single-cell sequencing. J Cancer 2019; 10(23):5883-5890. doi:10.7150/jca.32267. https://www.jcancer.org/v10p5883.htm
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Abstract

The previous cancer studies were difficult to reproduce since the tumor tissues were analyzed directly. But the tumor tissues were actually a mixture of different cancer cells. The transcriptome of single-cell was much robust than the transcriptome of a mixed tissue. The single-cell transcriptome had much smaller variance. In this study, we analyzed the single-cell transcriptome of 272 colorectal cancer (CRC) epithelial cells and 160 normal epithelial cells and identified 342 discriminative transcripts using advanced machine learning methods. The most discriminative transcripts were LGALS4, PHGR1, C15orf48, HEPACAM2, PERP, FABP1, FCGBP, MT1G, TSPAN1 and CKB. We further clustered the 342 transcripts into two categories. The upregulated transcripts in CRC epithelial cells were significantly enriched in Ribosome, Protein processing in endoplasmic reticulum, Antigen processing and presentation and p53 signaling pathway. The downregulated transcripts in CRC epithelial cells were significantly enriched in Mineral absorption, Aldosterone-regulated sodium reabsorption and Oxidative phosphorylation pathways. The biological analysis of the discriminative transcripts revealed the possible mechanism of colorectal cancer.

Keywords: colorectal cancer, single-cell sequencing, transcriptome, support vector machine, minimal redundancy maximal relevance, incremental feature selection


Citation styles

APA
Zhang, G.L., Pan, L.L., Huang, T., Wang, J.H. (2019). The transcriptome difference between colorectal tumor and normal tissues revealed by single-cell sequencing. Journal of Cancer, 10(23), 5883-5890. https://doi.org/10.7150/jca.32267.

ACS
Zhang, G.L.; Pan, L.L.; Huang, T.; Wang, J.H. The transcriptome difference between colorectal tumor and normal tissues revealed by single-cell sequencing. J. Cancer 2019, 10 (23), 5883-5890. DOI: 10.7150/jca.32267.

NLM
Zhang GL, Pan LL, Huang T, Wang JH. The transcriptome difference between colorectal tumor and normal tissues revealed by single-cell sequencing. J Cancer 2019; 10(23):5883-5890. doi:10.7150/jca.32267. https://www.jcancer.org/v10p5883.htm

CSE
Zhang GL, Pan LL, Huang T, Wang JH. 2019. The transcriptome difference between colorectal tumor and normal tissues revealed by single-cell sequencing. J Cancer. 10(23):5883-5890.

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